血管內(nèi)皮細(xì)胞有維持血管內(nèi)環(huán)境平衡的功能，血管內(nèi)皮細(xì)胞產(chǎn)生、分泌纖維蛋白凝集和分解系統(tǒng)的活化劑和抑制劑。另外它們調(diào)控血小板的粘附和聚集。內(nèi)皮細(xì)胞同時(shí)釋放分子，調(diào)控細(xì)胞增殖，控制血管壁功能。人臍靜脈內(nèi)皮細(xì)胞是應(yīng)用*普遍的進(jìn)行體外研究?jī)?nèi)皮細(xì)胞的細(xì)胞類型。臍靜脈內(nèi)皮細(xì)胞顯微鏡下形態(tài)如同鵝卵石，用vWF/Factor VIII 和 CD-31容易染色,可以攝入乙酰基化低密度脂蛋白。用IL-1或TNF-α預(yù)處理后，可選擇性地表達(dá)E-selectin和VCAM。
Sciencell研究實(shí)驗(yàn)室的臍靜脈內(nèi)皮細(xì)胞是從人臍帶組織分離得到的，原代凍存，冰凍運(yùn)輸。每管細(xì)胞密度超過(guò)5×10^5/ml。細(xì)胞特性經(jīng)vWF/Factor VIII和CD31 (P-CAM) 免疫熒光鑒定，能夠攝取DiI標(biāo)記乙酰化低密度脂蛋白(DiI-Ac-LDL)。本細(xì)胞經(jīng)檢測(cè)不含HIV-1、HBV、HCV、支原體、細(xì)菌、酵母菌和真菌。如采用ScienCell 實(shí)驗(yàn)室特制的培養(yǎng)基，可保證此細(xì)胞15倍增增殖。

推薦培養(yǎng)基：內(nèi)皮細(xì)胞培養(yǎng)基(ECM, Cat. #1001)

貨號(hào)	8000
產(chǎn)地	美國(guó)
縮寫(xiě)	HUVEC
規(guī)格	5 x 10^5 /1ml
用途	科研
儲(chǔ)存	液氮
運(yùn)輸	干冰
關(guān)鍵字	人臍靜脈內(nèi)皮細(xì)胞 HUVEC   人臍靜脈內(nèi)皮細(xì)胞 HUVEC  人臍靜脈內(nèi)皮細(xì)胞 HUVEC   人臍靜脈內(nèi)皮細(xì)胞 HUVEC  人臍靜脈內(nèi)皮細(xì)胞 HUVEC   人臍靜脈內(nèi)皮細(xì)胞 HUVEC  人臍靜脈內(nèi)皮細(xì)胞 HUVEC   人臍靜脈內(nèi)皮細(xì)胞 HUVEC
公司簡(jiǎn)介	合肥星肽生物科技有限公司作為專業(yè)的ScienCell中國(guó)代理商，專業(yè)經(jīng)營(yíng)實(shí)驗(yàn)室科研用原代細(xì)胞、原代細(xì)胞專用培養(yǎng)基、原代細(xì)胞無(wú)血清培養(yǎng)基、干細(xì)胞、干細(xì)胞培養(yǎng)基、干細(xì)胞無(wú)血清培養(yǎng)基的研究和開(kāi)發(fā)。合肥星肽生物科技有限公司始終致力于為客戶提供可靠的研究材料以及方便快捷的服務(wù)，對(duì)購(gòu)買項(xiàng)目的前期資料提供,中期合同保證,后期貨物跟蹤到 *終售后的確保項(xiàng)目準(zhǔn)確到位，都有相關(guān)專業(yè)人士進(jìn)行維護(hù),確保您在合肥合肥星肽生物科技有限公司公司獲得*上等服務(wù)！
聯(lián)系方式	何經(jīng)理，合肥星肽生物科技有限公司，Tel：0551-63802898      400-8702-898，                                                  E-mail：info@qingbio.com      http://www.27846.cn      QQ:514713116

1.) Guo W, Zhang D, Wang X, Kong W, Zhang Q, Zhang Y, Liu W. (2013) "Actin cytoskeleton modulates ADMA-induced NF-kappaB nuclear translocation and ICAM-1 expression in endothelial cells."?Med Sci Monit. 17: BR242

2.) Yu Y, Yang P, Chuah Q, Huang Y, Peng C, Lee Y, Chiu S. (2013) "Radiation-induced senescence in securin-deficient cancer cells promotes cell invasion involving the IL-6/STAT3 and PDGF-BB/PDGFR pathways."?Sci Rep. 3: 1675

3.) Chen G, Zhao L, Feng J, You G, Sun Q, Li P, Han D, Zhou H. (2013) "Validation of Reliable Reference Genes for Real-Time PCR in Human Umbilical Vein Endothelial Cells on Substrates with Different Stiffness."?PLoS One. 8: e67360

4.) Aisha AF, Ismail Z, Abu-Salah KM, Siddiqui JM, Ghafar G, Abdul Majid AM. (2013) "Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice"?BMC Complement Altern Med. 13: 168.?

5.) Pan Q, Pan H, Lou H, Xu Y, Tian L. (2013) "Inhibition of the angiogenesis and growth of Aloin in human colorectal cancer in vitro and in vivo."?Cancer Cell Int. 13: 69.

6.) Cheng HS, Sivachandran N, Lau A, Boudreau E, Zhao JL, Baltimore D, Delgado-Olguin P, Cybulsky MI, Fish JE. (2013) "MicroRNA-146 represses endothelial activation by inhibiting pro-inflammatory pathways."?EMBO Mol Med. 5: 1017-34.?

7.) Huiyong Z, Yong L, Didier M, Yu Z, Jing F, Rongyue C, Jingjing L. (2011) "Enhanced inhibition of murine prostatic carcinoma growth by immunization with or administration of viable human umbilical vein endothelial cells and CRM197."?Braz J Med Biol Res. 44: 140-8.

8.) Zhao Y, Yu L, Xu S, Qiu F, Fan Y, Fu G. (2011) "Down-regulation of connexin43 gap junction by serum deprivation in human endothelial cells was improved by (-)-Epigallocatechin gallate via ERK MAP kinase pathway."?Biochem Biophys Res Commun. 404: 217-22.

9.) Ghosh C, Gonzalez-Martinez J, Hossain M, Cucullo L, Fazio V, Janigro D, Marchi N. (2010) "Pattern of P450 expression at the human blood-brain barrier: roles of epileptic condition and laminar flow."?Epilepsia. 51: 1408-17.

10.) Fang W, Li H, Zhou L, Su L, Liang Y, Mu Y. (2010) "Effect of prostaglandin E1 on TNF-induced vascular inflammation in human umbilical vein endothelial cells."?Can J Physiol Pharmacol. 88: 576-83.

11.) Gao ZX, Huang DY, Li HX, Zhang LN, Lv YH, Cui HD, Zheng JH. (2010) "Scutellarin promotes in vitro angiogenesis in human umbilical vein endothelial cells."?Biochem Biophys Res Commun. 400: 151-6.

12.) Ou Y, Guo XL, Zhai L, Liu XY, Cheng YN. (2010) "TMPDP, a tetramethylpyrazine derivative, protects vascular endothelial cells from oxidation damage by hydrogen peroxide."?Pharmazie. 65: 755-9.

13.) Austin SA, Sens MA, Combs CK. (2009) "Amyloid precursor protein mediates a tyrosine kinase-dependent activation response in endothelial cells."?J Neurosci. 29: 14451-62.

14.) Cueni LN, Detmar M. (2009) "Galectin-8 interacts with podoplanin and modulates lymphatic endothelial cell functions."?Exp Cell Res. 315: 1715-23.?

15.) Li Y, Song YH, Li F, Yang T, Lu YW, Geng YJ. (2009) "MicroRNA-221 regulates high glucose-induced endothelial dysfunction."?Biochem Biophys Res Commun. 381: 81-3.

16.) Vigl B, Zgraggen C, Rehman N, Banziger-Tobler NE, Detmar M, Halin C. (2009) "Coxsackie- and adenovirus receptor (CAR) is expressed in lymphatic vessels in human skin and affects lymphatic endothelial cell function in vitro."?Exp Cell Res. 315: 336-47.

17.) Xiong YQ, Sun HC, Zhang W, Zhu XD, Zhuang PY, Zhang JB, Wang L, Wu WZ, Qin LX, Tang ZY. (2009) "Human hepatocellular carcinoma tumor-derived endothelial cells manifest increased angiogenesis capability and drug resistance compared with normal endothelial cells."?Clin Cancer Res. 15: 4838-46.?

18.) Cucullo L, Couraud PO, Weksler B, Romero IA, Hossain M, Rapp E, Janigro D. (2008) "Immortalized human brain endothelial cells and flow-based vascular modeling: a marriage of convenience for rational neurovascular studies."?J Cereb Blood Flow Metab. 28: 312-28.

19.) Yin W, Ghebrehiwet B, Weksler B, Peerschke EI. (2008) "Regulated complement deposition on the surface of human endothelial cells: effect of tobacco smoke and shear stress."?Thromb Res. 122: 221-8.

20.) Gupta GP, Perk J, Acharyya S, de Candia P, Mittal V, Todorova-Manova K, Gerald WL, Brogi E, Benezra R, Massagu? J. (2007) "ID genes mediate tumor reinitiation during breast cancer lung metastasis."?Proc Natl Acad Sci USA. 104: 19506-11.

21.) Ng ES, Cheng ZJ, Ellis A, Ding H, Jiang Y, Li Y, Hollenberg MD, Triggle CR. (2007) "Nitrosothiol stores in vascular tissue: Modulation by ultraviolet light, acetylcholine and ionomycin."?Eur J Pharmacol. 560: 183-92.?

22.) Yin W, Ghebrehiwet B, Weksler B, Peerschke EI. (2006) "Classical pathway complement activation on human endothelial cells."?Mol Immunol. 44: 2228-34.

23.) Ray RB, Basu A, Steele R, Beyene A, McHowat J, Meyer K, Ghosh AK, Ray R. (2004) "Ebola virus glycoprotein-mediated anoikis of primary human cardiac microvascular endothelial cells."?Virology. 321: 181-8.

24.) D?m?t?r E, Benzakour O, Griffin JH, Yule D, Fukudome K, Zlokovic BV. (2003) "Activated protein C alters cytosolic calcium flux in human brain endothelium via binding to endothelial protein C receptor and activation of protease activated receptor-1."?Blood. 101: 4797-801.

Question:

Greetings! I am the biosafety officer at our institution, and we've had a researcher order primary HUVEC cells from your company. Would you be able to let us know if you test the cultures/isolates for infectious agents or viruses and what agents you test for?

Answers:

Each cell strain tests negative for HIV, HBV and HCV. However, keep in mind that diagnostic tests are not necessarily 100% accurate, therefore, proper precautions must be taken to avoid inadvertent exposure. Always wear gloves and safety glasses when working with these materials. Never mouth pipette. We recommend following the universal procedures for handling products of human origin as the minimum precaution against contamination.